MERS-CoV virus-like particles produced in insect cells induce specific humoural and cellular imminity in rhesus macaques
Identifieur interne : 001162 ( Main/Exploration ); précédent : 001161; suivant : 001163MERS-CoV virus-like particles produced in insect cells induce specific humoural and cellular imminity in rhesus macaques
Auteurs : Chong Wang [République populaire de Chine] ; Xuexing Zheng [République populaire de Chine] ; Weiwei Gai [République populaire de Chine] ; Yongkun Zhao [République populaire de Chine] ; Hualei Wang [République populaire de Chine] ; Haijun Wang [République populaire de Chine] ; Na Feng [République populaire de Chine] ; Hang Chi [République populaire de Chine] ; Boning Qiu [République populaire de Chine] ; Nan Li [République populaire de Chine] ; Tiecheng Wang [République populaire de Chine] ; Yuwei Gao [République populaire de Chine] ; Songtao Yang [République populaire de Chine] ; Xianzhu Xia [République populaire de Chine]Source :
- Oncotarget [ 1949-2553 ] ; 2016.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux (immunologie), Anticorps neutralisants (immunologie), Coronavirus du syndrome respiratoire du Moyen-Orient (immunologie), Immunité cellulaire (immunologie), Immunité humorale (immunologie), Infections à coronavirus (immunologie), Macaca mulatta, Microscopie électronique à transmission, Modèles animaux de maladie humaine, Technique d'immunofluorescence indirecte, Technique de Western, Vaccins antiviraux (immunologie), Vaccins à pseudo-particules virales (immunologie).
- MESH :
- immunologie : Anticorps antiviraux, Anticorps neutralisants, Coronavirus du syndrome respiratoire du Moyen-Orient, Immunité cellulaire, Immunité humorale, Infections à coronavirus, Vaccins antiviraux, Vaccins à pseudo-particules virales.
- Animaux, Macaca mulatta, Microscopie électronique à transmission, Modèles animaux de maladie humaine, Technique d'immunofluorescence indirecte, Technique de Western.
English descriptors
- KwdEn :
- Animals, Antibodies, Neutralizing (immunology), Antibodies, Viral (immunology), Blotting, Western, Coronavirus Infections (immunology), Disease Models, Animal, Fluorescent Antibody Technique, Indirect, Immunity, Cellular (immunology), Immunity, Humoral (immunology), Insecta, Macaca mulatta, Microscopy, Electron, Transmission, Middle East Respiratory Syndrome Coronavirus (immunology), Vaccines, Virus-Like Particle (immunology), Viral Vaccines (immunology).
- MESH :
- chemical , immunology : Antibodies, Neutralizing, Antibodies, Viral, Vaccines, Virus-Like Particle, Viral Vaccines.
- immunology : Coronavirus Infections, Immunity, Cellular, Immunity, Humoral, Middle East Respiratory Syndrome Coronavirus.
- Animals, Blotting, Western, Disease Models, Animal, Fluorescent Antibody Technique, Indirect, Insecta, Macaca mulatta, Microscopy, Electron, Transmission.
Abstract
Middle East respiratory syndrome coronavirus (MERS-CoV) causes severe respiratory disease in humans with a case fatality rate of over 39%, and poses a considerable threat to public health. A lack of approved vaccine or drugs currently constitutes a roadblock in controlling disease outbreak and spread. In this study, we generated MERS-CoV VLPs using the baculovirus expression system. Electron microscopy and immunoelectron microscopy results demonstrate that MERS-CoV VLPs are structurally similar to the native virus. Rhesus macaques inoculated with MERS-CoV VLPs and Alum adjuvant induced virus-neutralizing antibodies titers up to 1:40 and induced specific IgG antibodies against the receptor binding domain (RBD), with endpoint titers reaching 1:1,280. MERS-CoV VLPs also elicited T-helper 1 cell (Th1)-mediated immunity, as measured by ELISpot. These data demonstrate that MERS-CoV VLPs have excellent immunogenicity in rhesus macaques, and represent a promising vaccine candidate.
Url:
DOI: 10.18632/oncotarget.8475
PubMed: 27050368
PubMed Central: 5355045
Affiliations:
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Le document en format XML
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wicri:level="3"><nlm:aff id="A2"> Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea> Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun</wicri:regionArea><placeName><settlement type="city">Changchun</settlement><region type="province">Jilin</region><region type="groupement">Dongbei</region></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="A4"> Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea> Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, 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for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun</wicri:regionArea><placeName><settlement type="city">Changchun</settlement><region type="province">Jilin</region><region type="groupement">Dongbei</region></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="A4"> Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea> Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou</wicri:regionArea><wicri:noRegion>Yangzhou</wicri:noRegion></affiliation></author><author><name sortKey="Yang, Songtao" sort="Yang, Songtao" uniqKey="Yang S" first="Songtao" last="Yang">Songtao Yang</name><affiliation wicri:level="3"><nlm:aff id="A2"> Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea> Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun</wicri:regionArea><placeName><settlement type="city">Changchun</settlement><region type="province">Jilin</region><region type="groupement">Dongbei</region></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="A4"> Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea> Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou</wicri:regionArea><wicri:noRegion>Yangzhou</wicri:noRegion></affiliation></author><author><name sortKey="Xia, Xianzhu" sort="Xia, Xianzhu" uniqKey="Xia X" first="Xianzhu" last="Xia">Xianzhu Xia</name><affiliation wicri:level="3"><nlm:aff id="A2"> Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea> Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun</wicri:regionArea><placeName><settlement type="city">Changchun</settlement><region type="province">Jilin</region><region type="groupement">Dongbei</region></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="A4"> Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea> 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macaques</title><author><name sortKey="Wang, Chong" sort="Wang, Chong" uniqKey="Wang C" first="Chong" last="Wang">Chong Wang</name><affiliation wicri:level="1"><nlm:aff id="A1"> College of Wildlife Resources, Northeast Forestry University, Harbin, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea> College of Wildlife Resources, Northeast Forestry University, Harbin</wicri:regionArea><wicri:noRegion>Harbin</wicri:noRegion></affiliation><affiliation wicri:level="3"><nlm:aff id="A2"> Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea> Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun</wicri:regionArea><placeName><settlement type="city">Changchun</settlement><region type="province">Jilin</region><region type="groupement">Dongbei</region></placeName></affiliation></author><author><name sortKey="Zheng, Xuexing" sort="Zheng, Xuexing" uniqKey="Zheng X" first="Xuexing" last="Zheng">Xuexing Zheng</name><affiliation wicri:level="3"><nlm:aff id="A2"> Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea> Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun</wicri:regionArea><placeName><settlement type="city">Changchun</settlement><region type="province">Jilin</region><region type="groupement">Dongbei</region></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="A3"> School of Public Health, Shandong University, Jinan, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea> School of Public Health, Shandong University, Jinan</wicri:regionArea><wicri:noRegion>Jinan</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A4"> Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea> Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou</wicri:regionArea><wicri:noRegion>Yangzhou</wicri:noRegion></affiliation></author><author><name sortKey="Gai, Weiwei" sort="Gai, Weiwei" uniqKey="Gai W" first="Weiwei" last="Gai">Weiwei Gai</name><affiliation wicri:level="3"><nlm:aff id="A2"> Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea> Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun</wicri:regionArea><placeName><settlement type="city">Changchun</settlement><region type="province">Jilin</region><region type="groupement">Dongbei</region></placeName></affiliation></author><author><name sortKey="Zhao, Yongkun" sort="Zhao, Yongkun" uniqKey="Zhao Y" first="Yongkun" last="Zhao">Yongkun Zhao</name><affiliation wicri:level="3"><nlm:aff id="A2"> Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea> Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun</wicri:regionArea><placeName><settlement type="city">Changchun</settlement><region type="province">Jilin</region><region type="groupement">Dongbei</region></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="A4"> Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea> Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou</wicri:regionArea><wicri:noRegion>Yangzhou</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Hualei" sort="Wang, Hualei" uniqKey="Wang H" first="Hualei" last="Wang">Hualei Wang</name><affiliation wicri:level="3"><nlm:aff id="A2"> Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea> Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun</wicri:regionArea><placeName><settlement type="city">Changchun</settlement><region type="province">Jilin</region><region type="groupement">Dongbei</region></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="A4"> Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea> Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou</wicri:regionArea><wicri:noRegion>Yangzhou</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Haijun" sort="Wang, Haijun" uniqKey="Wang H" first="Haijun" last="Wang">Haijun Wang</name><affiliation wicri:level="3"><nlm:aff id="A2"> Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea> Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun</wicri:regionArea><placeName><settlement type="city">Changchun</settlement><region type="province">Jilin</region><region type="groupement">Dongbei</region></placeName></affiliation></author><author><name sortKey="Feng, Na" sort="Feng, Na" uniqKey="Feng N" first="Na" last="Feng">Na Feng</name><affiliation wicri:level="3"><nlm:aff id="A2"> Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea> Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun</wicri:regionArea><placeName><settlement type="city">Changchun</settlement><region type="province">Jilin</region><region type="groupement">Dongbei</region></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="A4"> Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea> Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou</wicri:regionArea><wicri:noRegion>Yangzhou</wicri:noRegion></affiliation></author><author><name sortKey="Chi, Hang" sort="Chi, Hang" uniqKey="Chi H" first="Hang" last="Chi">Hang Chi</name><affiliation wicri:level="3"><nlm:aff id="A2"> Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea> Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun</wicri:regionArea><placeName><settlement type="city">Changchun</settlement><region type="province">Jilin</region><region type="groupement">Dongbei</region></placeName></affiliation></author><author><name sortKey="Qiu, Boning" sort="Qiu, Boning" uniqKey="Qiu B" first="Boning" last="Qiu">Boning Qiu</name><affiliation wicri:level="3"><nlm:aff id="A2"> Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea> Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun</wicri:regionArea><placeName><settlement type="city">Changchun</settlement><region type="province">Jilin</region><region type="groupement">Dongbei</region></placeName></affiliation></author><author><name sortKey="Li, Nan" sort="Li, Nan" uniqKey="Li N" first="Nan" last="Li">Nan Li</name><affiliation wicri:level="3"><nlm:aff id="A2"> Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea> Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun</wicri:regionArea><placeName><settlement type="city">Changchun</settlement><region type="province">Jilin</region><region type="groupement">Dongbei</region></placeName></affiliation></author><author><name sortKey="Wang, Tiecheng" sort="Wang, Tiecheng" uniqKey="Wang T" first="Tiecheng" last="Wang">Tiecheng Wang</name><affiliation wicri:level="3"><nlm:aff id="A2"> Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea> Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun</wicri:regionArea><placeName><settlement type="city">Changchun</settlement><region type="province">Jilin</region><region type="groupement">Dongbei</region></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="A4"> Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea> Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou</wicri:regionArea><wicri:noRegion>Yangzhou</wicri:noRegion></affiliation></author><author><name sortKey="Gao, Yuwei" sort="Gao, Yuwei" uniqKey="Gao Y" first="Yuwei" last="Gao">Yuwei Gao</name><affiliation wicri:level="3"><nlm:aff id="A2"> Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea> Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun</wicri:regionArea><placeName><settlement type="city">Changchun</settlement><region type="province">Jilin</region><region type="groupement">Dongbei</region></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="A4"> Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea> Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou</wicri:regionArea><wicri:noRegion>Yangzhou</wicri:noRegion></affiliation></author><author><name sortKey="Yang, Songtao" sort="Yang, Songtao" uniqKey="Yang S" first="Songtao" last="Yang">Songtao Yang</name><affiliation wicri:level="3"><nlm:aff id="A2"> Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea> Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun</wicri:regionArea><placeName><settlement type="city">Changchun</settlement><region type="province">Jilin</region><region type="groupement">Dongbei</region></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="A4"> Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea> Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou</wicri:regionArea><wicri:noRegion>Yangzhou</wicri:noRegion></affiliation></author><author><name sortKey="Xia, Xianzhu" sort="Xia, Xianzhu" uniqKey="Xia X" first="Xianzhu" last="Xia">Xianzhu Xia</name><affiliation wicri:level="3"><nlm:aff id="A2"> Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea> Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun</wicri:regionArea><placeName><settlement type="city">Changchun</settlement><region type="province">Jilin</region><region type="groupement">Dongbei</region></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="A4"> Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea> Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou</wicri:regionArea><wicri:noRegion>Yangzhou</wicri:noRegion></affiliation></author></analytic><series><title level="j">Oncotarget</title><idno type="eISSN">1949-2553</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Neutralizing (immunology)</term><term>Antibodies, Viral (immunology)</term><term>Blotting, Western</term><term>Coronavirus Infections (immunology)</term><term>Disease Models, Animal</term><term>Fluorescent Antibody Technique, Indirect</term><term>Immunity, Cellular (immunology)</term><term>Immunity, Humoral (immunology)</term><term>Insecta</term><term>Macaca mulatta</term><term>Microscopy, Electron, Transmission</term><term>Middle East Respiratory Syndrome Coronavirus (immunology)</term><term>Vaccines, Virus-Like Particle (immunology)</term><term>Viral Vaccines (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antiviraux (immunologie)</term><term>Anticorps neutralisants (immunologie)</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (immunologie)</term><term>Immunité cellulaire (immunologie)</term><term>Immunité humorale (immunologie)</term><term>Infections à coronavirus (immunologie)</term><term>Macaca mulatta</term><term>Microscopie électronique à transmission</term><term>Modèles animaux de maladie humaine</term><term>Technique d'immunofluorescence indirecte</term><term>Technique de Western</term><term>Vaccins antiviraux (immunologie)</term><term>Vaccins à pseudo-particules virales (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Neutralizing</term><term>Antibodies, Viral</term><term>Vaccines, Virus-Like Particle</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antiviraux</term><term>Anticorps neutralisants</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term><term>Immunité cellulaire</term><term>Immunité humorale</term><term>Infections à coronavirus</term><term>Vaccins antiviraux</term><term>Vaccins à pseudo-particules virales</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Coronavirus Infections</term><term>Immunity, Cellular</term><term>Immunity, Humoral</term><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Blotting, Western</term><term>Disease Models, Animal</term><term>Fluorescent Antibody Technique, Indirect</term><term>Insecta</term><term>Macaca mulatta</term><term>Microscopy, Electron, Transmission</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Macaca mulatta</term><term>Microscopie électronique à transmission</term><term>Modèles animaux de maladie humaine</term><term>Technique d'immunofluorescence indirecte</term><term>Technique de Western</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Middle East respiratory syndrome coronavirus (MERS-CoV) causes severe respiratory disease in humans with a case fatality rate of over 39%, and poses a considerable threat to public health. A lack of approved vaccine or drugs currently constitutes a roadblock in controlling disease outbreak and spread. In this study, we generated MERS-CoV VLPs using the baculovirus expression system. Electron microscopy and immunoelectron microscopy results demonstrate that MERS-CoV VLPs are structurally similar to the native virus. Rhesus macaques inoculated with MERS-CoV VLPs and Alum adjuvant induced virus-neutralizing antibodies titers up to 1:40 and induced specific IgG antibodies against the receptor binding domain (RBD), with endpoint titers reaching 1:1,280. MERS-CoV VLPs also elicited T-helper 1 cell (Th1)-mediated immunity, as measured by ELISpot. 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last="Gao">Yuwei Gao</name><name sortKey="Gao, Yuwei" sort="Gao, Yuwei" uniqKey="Gao Y" first="Yuwei" last="Gao">Yuwei Gao</name><name sortKey="Li, Nan" sort="Li, Nan" uniqKey="Li N" first="Nan" last="Li">Nan Li</name><name sortKey="Qiu, Boning" sort="Qiu, Boning" uniqKey="Qiu B" first="Boning" last="Qiu">Boning Qiu</name><name sortKey="Wang, Chong" sort="Wang, Chong" uniqKey="Wang C" first="Chong" last="Wang">Chong Wang</name><name sortKey="Wang, Haijun" sort="Wang, Haijun" uniqKey="Wang H" first="Haijun" last="Wang">Haijun Wang</name><name sortKey="Wang, Hualei" sort="Wang, Hualei" uniqKey="Wang H" first="Hualei" last="Wang">Hualei Wang</name><name sortKey="Wang, Hualei" sort="Wang, Hualei" uniqKey="Wang H" first="Hualei" last="Wang">Hualei Wang</name><name sortKey="Wang, Tiecheng" sort="Wang, Tiecheng" uniqKey="Wang T" first="Tiecheng" last="Wang">Tiecheng Wang</name><name sortKey="Wang, Tiecheng" sort="Wang, Tiecheng" uniqKey="Wang T" first="Tiecheng" last="Wang">Tiecheng Wang</name><name sortKey="Xia, Xianzhu" sort="Xia, Xianzhu" uniqKey="Xia X" first="Xianzhu" last="Xia">Xianzhu Xia</name><name sortKey="Xia, Xianzhu" sort="Xia, Xianzhu" uniqKey="Xia X" first="Xianzhu" last="Xia">Xianzhu Xia</name><name sortKey="Yang, Songtao" sort="Yang, Songtao" uniqKey="Yang S" first="Songtao" last="Yang">Songtao Yang</name><name sortKey="Yang, Songtao" sort="Yang, Songtao" uniqKey="Yang S" first="Songtao" last="Yang">Songtao Yang</name><name sortKey="Zhao, Yongkun" sort="Zhao, Yongkun" uniqKey="Zhao Y" first="Yongkun" last="Zhao">Yongkun Zhao</name><name sortKey="Zhao, Yongkun" sort="Zhao, Yongkun" uniqKey="Zhao Y" first="Yongkun" last="Zhao">Yongkun Zhao</name><name sortKey="Zheng, Xuexing" sort="Zheng, Xuexing" uniqKey="Zheng X" first="Xuexing" last="Zheng">Xuexing Zheng</name><name sortKey="Zheng, Xuexing" sort="Zheng, Xuexing" uniqKey="Zheng X" first="Xuexing" last="Zheng">Xuexing Zheng</name><name sortKey="Zheng, Xuexing" sort="Zheng, Xuexing" uniqKey="Zheng X" first="Xuexing" last="Zheng">Xuexing Zheng</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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